If you want higher quality results with lower overall costs in your laboratory, a focus on filtration could be just what you are looking for.
Matching the right filter for the right process can extend column life, reduce instrument maintenance costs, and cut the need for time-consuming retests. What’s more, it will also protect sample integrity, giving you greater confidence in your results.
At Cytiva, we believe quality science starts with getting the basics right. So, here are our top tips for matching the right filter to the right sample preparation workflow:
- Think about coarse particulates in the dissolution media. For high levels of particulates, choose a depth filter that only contains glass fibre. For low volumes, go for a membrane filter.
- Standard depth filter not providing a clear filtrate? Or is the membrane filter getting clogged? Try a combined depth and membrane filter.
- Chemical compatibly matters. Validate your filter media to the pH, ionic strength, and surfactant of the sample.
- Reduce the workload of validation by using membranes with a broad chemical compatibility, like regenerated cellulose (RC), Hydrophilic PTFE (H-PTFE) or Polyvinylidene Fluoride (PVDF).
- Have you tried RC and glass fibre membranes? Their lower adsorption properties help labs avoid the quantification errors that can happen when dissolved API material is adsorbed into the filter.
- Sample and test multiple membrane types to determine optimal detection performance. Remember, when analytes are detected at the same wavelength as the API or any extractables in the sample, it affects sample integrity
- Using filter materials with low levels of extractables, such as RC or H-PTFE, can help avoid interference
- Using semi- or fully-automated systems? Make sure the syringe filters’ polypropylene housing is dimensionally compatible with the instrument for smooth transportation from the storage turntable to the filtration site
Pharma labs worldwide want to increase efficiency, accelerate workflows, and have confidence in their results. Our advice? Start with the basics and build your foundation for high quality science — at lower overall costs.
Want to know more about filter selection?
Download our deep dive article, Filtering Out Inefficiencies in Dissolution Testing, to learn how the right filter can save pharma laboratories time and money