July 06, 2018

Superose™ 12 columns replacement

By Cytiva

On Dec. 15 this year, Superose 12 columns will be discontinued. They can already be replaced by our new-generation size exclusion chromatography (SEC) columns—Superdex Increase and Superose Increase.

The new-generation SEC columns, deliver excellent resolution and optimized run times for small-scale preparative purification and analysis.

Four resins are available that can be used to replace Superose 12 resin.

Which new-generation SEC column to choose?

Below is an overview of GE’s new-generation SEC resins that can be used as a replacement for Superose 12 resin, including application examples. These resins are available in three different column sizes.

Superdex 30 Increase Mr ~ 100 to 7000

  • Peptides
  • Oligosaccharides
  • Separation of polyphenols (TCM*)
  • Other small biomolecules

Discover Superdex 30 Increase >>

Superdex 75 Increase Mr ~ 3000 to 70 000

  • Proteins, enzymes, tagged proteins
  • Polysaccharides
  • Separation of polyphenols (TCM)

Discover Superdex 75 Increase >>

Superdex 200 Increase Mr ~ 10 000 to 600 000

  • mAb and other antibodies
  • Proteins, enzymes, tagged proteins
  • Polysaccharides

Discover Superdex 200 Increase >>

Superose 6 Increase Mr ~ 5000 to 5 000 000*

  • Large proteins
  • Protein complexes

Discover Superose 6 Increase >>

TCM = Traditional Chinese Medicine
* The image is reproduced from Wikimedia Commons (https://en.wikipedia.org/wiki/File:Protein_AP2A1_PDB_1gw5.png) and is licensed under the Creative Commons Attribution-Share Alike 3.0 Unported license.

The new-generation SEC resins cover the fractionation range for Superose™ 12 as shown in the picture below.

Approximate fractionation ranges of Superose 12 and its new generation replacement columns

Example of a protein mix separation

The chromatogram below shows the separation of a protein mix with Superose 12 and with the four replacement SEC resins. The table shows which conclusions can be drawn when it comes to suitability of these columns for purifying each protein from the protein mix.

Guide to selecting a replacement resin for Superose 12. Chromatograms showing separation of eight molecules on Superdex 30 Increase, Superdex 75 Increase, Superdex 200 Increase, Superose 6 Increase, and Superose 12. Peak numbers correspond to peaks listed in the table below. 

If you need further guidance, feel free to contact us

Contact us, and we will make our best to support you for transitioning from using Superose 12 to using our new-generation SEC columns.