Blotting standards and reagents

Cy3 and Cy5 maleimide mono functional dyes are especially suitable for highly selective peptide, oligonucleotide and protein labeling via thiol maleimide coupling. 

Fluorescence detection is a direct Western blotting method in which a secondary antibody is conjugated to a fluorophore, avoiding the need for ancillary detection reagents. Different fluorophores are available for signal detection at either visible light or near-infrared (NIR) wavelengths. Amersham CyDye 700 and 800 secondary antibodies are labeled with NIR fluorophores that emit light at wavelengths of 700 nm or 800 nm. By using Amersham CyDye 700 and 800 secondary antibodies together with a pair of appropriate rabbit or mouse primary antibodies, you can perform multiplexed experiments. Key benefits:

During the Western blotting process, non-specific binding of antibodies to the membrane is detrimental to the specificity and sensitivity of the assay. To overcome this challenge, it is essential to block parts of the membrane not already occupied by proteins. The Amersham ECL blocking agent can be used for blocking Amersham Protran nitrocellulose (NC) and Amersham Hybond polyvinylidene fluoride (PVDF) membranes when used along with the ECL chemiluminescence detection reagents, such as Amersham ECL, ECL start, ECL Prime, and ECL Select. Each pack contains 40 g of blocking agent which is sufficient for at least 20 mini blots.

Amersham ECL detection reagent uses enhanced luminol-based detection suitable for all routine confirmatory Western blotting experiments. Proven reliability:

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Amersham ECL Horseradish peroxidase (HRP) conjugated antibodies are highly species specific antibodies optimized for use with Amersham ECL Western blotting detection reagents.

Fluorescence detection is a direct Western blotting method in which a secondary antibody is conjugated to a fluorophore, avoiding the need for ancillary detection reagents. Amersham Cy3 and Cy5 secondary antibodies are labeled with near-infrared (NIR) fluorophores that emit light at wavelengths of 570 and 670 nm. By using Amersham Cy3 and Cy5 secondary antibodies together with a pair of appropriate rabbit or mouse primary antibodies, you can perform multiplexed experiments. Key benefits:

Amersham ECL Plex Fluorescent Rainbow markers are optimized for use with Amersham CyDye products. The markers will provide:

Combination packs include the following, sufficient for at least 1000 cm 2 of membrane:

During the Western blotting process, non-specific binding of antibodies to the membrane is detrimental to the specificity and sensitivity of the assay. To overcome this challenge, it is essential to block parts of the membrane not already occupied by proteins. The Amersham ECL Prime blocking agent can be used for blocking Amersham Protran nitrocellulose (NC) and Amersham Hybond polyvinylidene fluoride (PVDF) membranes when used along with the ECL chemiluminescence detection reagents, such as Amersham ECL, ECL start, ECL Prime, ECL Select, and ECL Plex. Each pack contains 40 g of blocking agent, which is sufficient for at least 20 mini blots.

Amersham ECL Prime is a highly sensitive chemiluminescent detection reagent and is characterized by extremely stable signal emission. Key benefits:

Rainbow molecular weight markers enhanced to enable faster and simpler identification of proteins on SDS-polyacrylamide gels.

ECL Select™ reagent is the most sensitive chemiluminescent Western blotting detection reagent in the ECL™ product family. Key product features:

Chemiluminescent detection of medium- and high-expression proteins, with a long lasting signal that provides a longer time window for detection.