Fed-batch applications

This HyClone™ cell culture supplement has been developed for recombinant protein production with various cell lines including Chinese hamster ovary (CHO) cell line.

General culture recommendations

Supplement 100 to 200 mL/L, one or more times, starting during early- to mid-growth phase. This is expected to be day 2 to 4. Start the culture at a reduced volume according to anticipated feed volumes. Continue supplementation daily until the final volume is reached. It is recommended to monitor L- glutamine separately and supplement as needed.

Dry powdered medium milling equivalency study

This study compares pin milling of powdered HyClone™ cell culture media and supplements at two manufacturing sites. Detailed physical, chemical, and biological data, including cell growth, are provided.