Fed-batch applications

This HyClone cell culture supplement has been developed for high-yield recombinant protein production when using various Chinese hamster ovary (CHO) cell lines.

Composition of HyClone Cell Boost 7b cell culture media supplement

Cell Boost 7b CHO supplement has an alkaline pH and is a concentrated solution of amino acids. Like Cell Boost 7a, this media supplement does not contain any growth factors (such as insulin), peptides, hydrolysates, phenol red, or 2-mercaptoethanol ensuring batch-to-batch consistency and increased cell culture process efficiency.

Applications data for CHO bioreactor cultures using HyClone Cell Boost supplements

A recent study compared cell growth and recombinant protein production of multiple Chinese hamster ovary (CHO) cell clones when cultured in HyClone ActiPro basal medium and Cell Boost 7a and 7b media supplements. Results from shake flask cultures showed high viable cell densities and protein production. In addition, scalability of the culture process was demonstrated in 2 and 50 L bioreactor cultures. Cell density and protein production were shown to be comparable between scales. Protein yields reached as high as 5 g/L in shake flasks, and as high as 5.5 g/L in bioreactor cultures.

Dry powdered medium milling equivalency study

This study compares pin milling of powdered HyClone cell culture media and supplements at two manufacturing sites. Detailed physical, chemical, and biological data, including cell growth, are provided.