The animals are fed an enriched diet to ensure proper nutrition and are fasted prior to bleeding to reduce lipid concentration. The horses are bled in an enclosed facility that is specially equipped for aseptic collection. Coggins tests for equine infectious anemia are routinely performed on each horse.

Donor Equine Serum is filtered through three sequential 100 nm (0.1 μm) pore size-rated filters. Before dispensing, each lot of serum is pooled using true pool technology to ensure uniformity and consistency between bottles.

Additional treatments available including heat-inactivation and irradiation. Additional testing, including EMEA-conforming testing, is also available.