A highly stable sucrose polymer with an average molecular weight of 400 000, suitable for isolating eukaryotic cells and organelles with minimal damage.

  • For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
  • Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved.
  • Better osmotic properties than sucrose.
  • Preserves functional and morphological integrity.
  • Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
  • Does not penetrate biological membranes.
  • Serves as the raw material for preparation of Ficoll-Paque gradients.
  • Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.

High density without increased osmotic pressure

Ficoll PM400 is a synthetic neutral, highly-branched hydrophilic polymer of sucrose with an average molecular weight of 400 000. It has long been used to form density gradients for separating and isolating eukaryotic cells, organelles and bacterial cells, as a stabilizing agent, and as a preparation medium for isolating mononuclear cells.

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Product Product Name Price
17030005 Ficoll PM400 5 kg 6,942.00 USD Add to cart


17030050 Ficoll PM400 (Dry Powder) 500 g 1,085.00 USD Add to cart


17030008 Ficoll PM400 40 kg 40,500.00 USD Add to cart


17030010 Ficoll PM400 (Dry Powder) 100 g 260.00 USD Add to cart


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