HiTrap rProtein A FF columns are prepacked with rProtein A Sepharose Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.

  • Quick, convenient preparative purification of monoclonal antibodies.
  • Well-established protein A-based resin used in many approved MAb processes.
  • Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  • Simple operations with a syringe, pump, or high-performance chromatography system, such as ÄKTA design.

The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.

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Product Product Name Price
17507901 HiTrap rProtein A FF, 5 x 1 ml 5 × 1 mL 881.00 USD Add to cart


28946489 HiTrap rProtein A FF 100 x 1 ml 100 x 1 mL Discontinued
17508002 HiTrap rProtein A FF, 5 x 5 ml 5 × 5 mL 3,533.00 USD Add to cart


17508001 HiTrap rProtein A FF, 1 x 5 ml 1 × 5 mL 867.00 USD Add to cart


17507902 HiTrap rProtein A FF, 2 x 1 ml 2 × 1 mL 428.00 USD Add to cart


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