HiTrap Blue HP is prepacked with Blue Sepharose High Performance for capture of a wide range of biomolecules, including: albumin, interferon, a broad range of nucleotide-dependent enzymes, α2-macroglobulin, coagulation factors, and related proteins.
- Can be used for depletion of albumin from serum.
- Cibacron Blue F3G-A, the ligand, is covalently attached to the highly cross-linked agarose resin and is stable over a pH range of 4 to 12 for use and 3 to 13 for cleaning.
- The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA design or FPLC System.
Blue Sepharose High Performance is a group specific adsorbent with affinity for a wide variety of enzymes. The dye ligand, Cibacron Blue F3G-A, is covalently attached to the matrix via the triazine part of the dye molecule. Some proteins interact biospecifically with the dye due to its structural similarity with nucleotide cofactors, while others, such as albumin and interferon, bind in a less specific manner by electrostatic and/or hydrophobic interactions with the aromatic anionic ligand.
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