Use these columns for IgG purification from cow, sheep, horse, rat, mouse, and human sources, including ascites, serum, and cell culture supernatants.

HiTrap Protein G HP columns deliver high-performance, high-resolution purifications with a binding capacity of 25 mg IgG/mL resin and fast kinetics. Columns are stable over a wide pH range – operational pH 3 to 9, and pH 2 to 10 for cleaning.

Successful use in published research

Protein G HiTrap columns are relied upon for a variety of research applications, including:

• Purification of primary antibody from rabbit for research on human lung cells
• mAb purification from ascites for research on the protein C anticoagulant pathway
• Purification of IgG antifilaggrin autoantibodies from human sera

Other formats of Protein G Sepharose HP resin

Protein G Sepharose High Performance resin is also available in the following formats:

• Protein G HP SpinTrap spin columns, for small sample purification using a microcentrifuge
• Protein G HP MultiTrap 96-well plates, for high-throughput screening and small-scale purification using centrifuge or vacuum equipment