NHS Sepharose High Performance

  • Simple, small-scale enrichment of target proteins using pull-down technique.
  • Antibody or capturing molecule covalently immobilized via free NH2 groups.
  • Simple coupling, carried out near neutral pH.
  • Fast and flexible protocol for the capture step, with elution conditions formatted for both electrophoresis and LC-MS analysis workflows
  • Easy scale-up to HiTrap NHS-activated HP Columns
  • The kit contains bulk resin and empty SpinTrap columns to allow for batch coupling
  • NHS HP SpinTrap consists of NHS-activated Sepharose High Performance, a medium to which molecules with primary amino groups can bind covalently, and 24 empty spin columns
  • NHS HP SpinTrap includes empty SpinTrap columns and NHS-activated Sepharose High Performance medium in bulk to allow for batch coupling. Proteins of interest are captured and enriched via an antibody or other molecule (e.g., protein) with desired affinity coupled to the medium through the primary amine. NHS Sepharose High Performance Medium is delivered in 100% isopropanol to protect the active groups. NHS HP SpinTrap columns are used with a standard microcentrifuge
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Product Product Name Price
28903128 NHS HP SpinTrap Temporarily unavailable

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