The Glutathione S-transferase (GST) Gene Fusion System from Cytiva is a versatile system for the expression, purification, and detection of GST-tagged proteins produced in E. coli.
- Enables the site-specific cleavage of fusion proteins containing an accessible Factor Xa protease recognition sequence.
- Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow.
Factor Xa, purified from bovine plasma, is a protease used to digest fusion proteins prepared from pGEX vectors containing the recognition sequence for factor Xa (pGEX-3X, pGEX-5X-1, pGEX-5X-2, and pGEX-5X-3). It specifically cleaves following the tetrapeptide Ile-Glu-Gly-Arg.
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