HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
- High binding capacity, approx. 40 mg/mL resin.
- Negligible leakage of Ni2+.
- Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications
Read moreProduct | Product Name | Price | ||
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17525501 | HisTrap FF | 5 × 5 mL | 677.00 USD |
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17531901 | HisTrap FF | 5 × 1 mL | 197.05 USD |
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17531902 | HisTrap FF | 100 x 1 mL | Discontinued, replaced by HisTrap FF | |
17525502 | HisTrap FF | 100 x 5 mL | Discontinued, replaced by HisTrap FF |
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