These PreDictor™ RoboColumn™ minicolumns for high-throughput process development (HTPD) are prepacked with Ni Sepharose™ excel affinity resin. For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

• Supports HTPD — enables parallel screening of chromatographic conditions using a miniaturized column format and robotic workstation.
• Miniaturized column format — facilitates screening of a wide range of parameters with small sample consumption.
• Reduced experimental time — automation and parallel screening minimize labor and save time.
• Increased process understanding — high-throughput workflow allows investigation of enlarged experimental workspace for better process understanding.
• Packed with Ni Sepharose™ excel resin — designed for his-tagged protein purification with minimized Ni-leakage and maximized protein recovery when samples contain stripping agents.