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DEAE Sepharose Fast Flow anion exchange chromatography resin

DEAE Sepharose Fast Flow is a weak anion exchange chromatography resin (medium) for fast protein purification.

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ID	Product Name
17070905	DEAE Sepharose Fast Flow, 10 L
17070910	DEAE Sepharose Fast Flow, 25 mL
17070901	DEAE Sepharose Fast Flow, 500 mL
17070960	DEAE Sepharose Fast Flow, 60 L

DEAE Sepharose Fast Flow is a weak anion exchange chromatography resin (medium) for fast protein purification.

Well-proven weak anion exchanger developed for industrial downstream processes.
Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals.
High chemical stability allows for well proven CIP and sanitization protocols.
Hydrophilic base matrix ensures low levels of non-specific binding, and low levels of host cell-derived impurities in the elution pool.
Fulfills industrial demands for security of supply, robust performance, and regulatory support.

Streamlined scale-up

This resin is part of the Sepharose Fast Flow ion exchange platform, which is well established in industrial downstream processing. Composed of crosslinked 6% agarose beads with diethylaminoethyl (DEAE) weak anion exchange groups, the resin has high chemical stability, allowing well-proven cleaning-in-place (CIP) and sanitization protocols.

The highly crosslinked agarose resin enables high flow rates and high productivity. Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, for example PreDictor 96-well plates, and PreDictor RoboColumns™, and HiTrap columns.

Successful use – research scale

DEAE Sepharose Fast Flow resin is used in a variety of research applications, including:

Separation ofPI 3-Kinases from guinea pig neutrophils
Purification of myosin phosphatase from chicken smooth muscle

Compatible empty columns – research scale

Cytiva's solutions include a variety of empty columns for research scale. This DEAE resin is compatible with two of these lines, Tricorn and XK columns.

Compatible empty columns – bioprocess scale

For process-scale applications for this DEAE resin, Cytiva recommends AxiChrom columns. DEAE Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.

Prepacked columns – research scale

This weak anion exchange resin is available in the following formats:

HiScreen DEAE FF for scale up and process development
HiPrep DEAE FF columns for convenient scale-up

Prepacked columns – bioprocess scale

Prepacked ReadyToProcess columns for bioprocess applications are available on request.

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Product	Product Name	Pack size All 10 L 25 mL 500 mL 60 L	Price
17070901	DEAE Sepharose Fast Flow, 500 mL	500 mL	581.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
17070905	DEAE Sepharose Fast Flow, 10 L	10 L	On Request	Get Quote
17070910	DEAE Sepharose Fast Flow, 25 mL	25 mL	76.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
17070960	DEAE Sepharose Fast Flow, 60 L	60 L	On Request	Get Quote

DEAE Sepharose Fast Flow anion exchange chromatography resin

DEAE Sepharose Fast Flow anion exchange chromatography resin

Streamlined scale-up

Successful use – research scale

Compatible empty columns – research scale

Compatible empty columns – bioprocess scale

Prepacked columns – research scale

Prepacked columns – bioprocess scale

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DEAE Sepharose Fast Flow anion exchange chromatography resin

DEAE Sepharose Fast Flow anion exchange chromatography resin

Streamlined scale-up

Successful use – research scale

Compatible empty columns – research scale

Compatible empty columns – bioprocess scale

Prepacked columns – research scale

Prepacked columns – bioprocess scale

Select your model

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