The functional groups of this SP resin are coupled to the matrix via chemically stable ether linkages and remain charged over the entire working pH range. They also maintain high capacity for cation exchange separations.

Successful use in published research

SP Sepharose Fast Flow resin, which is packed into HiPrep columns, is used in a variety of research applications, including:

• Purification of pro-form lipase from Rhizopus oryzae in Pichia pastoris
• Purification of solid-phase PEGylated recombinant human keratinocyte growth factor 1
• Development of a lentiviral vector platform for production of bioengineered recombinant coagulation factor VIII

Other SP Sepharose Fast Flow formats

This cation resin is available in the following formats:

• Bulk resin SP Sepharose Fast Flow
• HiTrap SP FF columns convenient for easy use with a syringe, peristaltic pump, or chromatography system
• HiScreen SP FF columns for method and process development