The column package allows you to combine affinity with size exclusion chromatography to achieve very high purity of human IgG.

  • Contains columns suitable for a successful 2-step protocol of human antibodies: HiTrap MabSelect PrismA (1 × 1 mL) and HiPrep 16/60 Sephacryl S-300 HR.
  • Includes instruction with guidance and tips to help you quickly get started.
  • HiTrap MabSelect PrismA column is prepacked with MabSelect PrismA resin for preparative purification of monoclonal and polyclonal antibodies from serum and cell culture supernatant, providing excellent purity in one step.
  • You obtain final high purity with the HiPrep 16/60 Sephacryl S-300 HR size exclusion column, which removes antibody aggregates and/or fragments.

Protein A affinity chromatography captures your antibody

The kit includes a HiTrap column prepacked with MabSelect PrismA resin containing a protein A-derived ligand produced in E. coli. The ligand has been specially engineered to create an affinity resin with enhanced alkali and protease stability. The specificity of binding to the Fc region of IgG is similar to conventional Protein A and provides you with excellent purification in one step.

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Product Product Name Price
29497629 Antibody column package, modern Protein A (2-step purification protocol) 1 kit 929.00 USD Add to cart


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