The column package allows you to combine affinity with size exclusion chromatography to obtain highly pure his-tagged proteins.

  • Contains a HisTrap HP column 1 mL prepacked with Ni Sepharose High Performance (HP) affinity resin for preparative purification of his-tagged proteins.
  • You achieve final purity with HiPrep 16/60 Sephacryl S-200 HR size exclusion chromatography column, which removes any remaining trace of impurities or closely related substances and imidazole.
  • Includes instructions with guidance and tips showing you how to get started.

Nickel ion chelating groups for efficient capture of your his-tagged proteins

Ni Sepharose HP affinity resin consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with nickel, which selectively retains proteins with exposed histidine groups. Ni Sepharose HP has a binding capacity that exceeds 40 mg/mL, resulting in high protein recovery.

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Product Product Name Price
29497630 Tagged column package, His (2-step purification protocol) 1 kit 702.00 USD Add to cart Get Quote

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