Key applications include:

• isolation and purification of IgG from serum, ascites, and tissue culture media
• monoclonal antibody purification
• isolation of antibody-ligand immune complexes
• immunoprecipitation protocols

The recombinant protein G ligand binds strongly to the Fc-region from a variety of mammalian species. In addition, many antibodies also interact via the Fab region with a low affinity site on protein G. The ligand is immobilized via a multipoint coupling to the Sepharose 4 Fast Flow agarose matrix to minimize ligand leakage.

Protein G Sepharose 4 Fast Flow resin is part of Cytiva's BioProcess media (resins) range, which ensures security of supply and comprehensive technical and regulatory support.

Successful use in published research

This resin is used in a variety of affinity chromatography applications, including:

• purification of the immunoglobulin fraction from rabbits
• immunoprecipitation (IP) Scientific Method Guide

Bioprocess applications

Protein G Sepharose Fast Flow resin is used in specific separation applications where broad binding specificity is required. An example is the scavenging of bovine IgG from serum prior to cell culture.

Compatible empty columns – research and bioprocess scale

This Fast Flow affinity chromatography resin can be packed into Cytiva's Tricorn, XK, and HiScale columns.

For process-scale applications, Cytiva recommends AxiChrom columns. Protein G Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.

Compatible empty columns – bioprocess scale

For process-scale applications, Cytiva recommends AxiChrom columns. Protein G Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.

Convenient prepacked columns – research use

This resin is available prepacked into Protein G GraviTrap columns for use with gravity flow.

Other Cytiva products for antibody purification

Life Sciences solutions from Cytiva include a wide array of protein A- and protein G-based resins and columns for use with gravity flow, centrifugation, pumps, or chromatography systems.