This immobilized metal affinity chromatography (IMAC) resin is designed to purify a wide range of proteins. It is supplied free of metal ions (uncharged), allowing the user to charge it with the most appropriate metal ion for purification of a target protein.

Streamlined scale up

Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward.

Successful use – research scale

Chelating Sepharose Fast Flow is an established tool for a range of research applications. For instance, this metal chelate affinity chromatography resin has been used to purify his-tagged proteins for the following purposes:

• Map the spatial structure of the dimeric transmembrane domain of the growth factor receptor ErbB2
• Identify distinct PIases with proven enzyme activity in E. coli

Compatible empty columns – research scale

Cytiva's solutions include a variety of empty columns for research scale. Chelating Sepharose Fast Flow is compatible with two of these lines, Tricorn and XK columns.

Compatible empty columns – bioprocess scale

For process-scale applications, Cytiva recommends AxiChrom columns. Chelating Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.

Prepacked Chelating Sepharose Fast Flow columns – research scale

This resin is available as HiTrap columns for use with a syringe, a pump, or a chromatography system.

Prepacked Chelating Sepharose Fast Flow columns – bioprocess scale

Prepacked ReadyToProcess columns for bioprocess applications are available on request, eliminating the need for column packing and cleaning validation.