IMAC Sepharose™ 6 Fast Flow resin
IMAC Sepharose 6 Fast Flow is an uncharged IMAC resin for purifying proteins and peptides with affinity for metal ions in batch mode or when scale up is needed.
IMAC Sepharose™ 6 Fast Flow resin
IMAC Sepharose 6 Fast Flow is an uncharged IMAC resin for purifying proteins and peptides with affinity for metal ions in batch mode or when scale up is needed.
Pack size
Overview
- Supplied uncharged, for customized metal ion charging and optimized selectivity.
- Suitable for purification of histidine-tagged (his-tagged) proteins when nickel is not the best choice of metal ion.
- Based on well-established Sepharose Fast Flow resins.
- High chemical stability enables proven CIP and sanitization protocols.
- Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
- Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
Uncharged IMAC resin for his-tagged and untagged proteins
IMAC Sepharose 6 Fast Flow is a member of Cytiva's range of immobilized metal ion affinity chromatography (IMAC) resins, designed for high-quality capture and intermediate native or recombinant protein purification. This resin is commonly used to purify his-tagged proteins, but also untagged recombinant or native proteins.
Resin is composed of cross-linked 6% agarose beads modified with a chelating ligand, which is immobilized to the base matrix. The highly crosslinked resin enables high flow rates and productivity. Resin is supplied uncharged, allowing the user to charge with the metal ion that has optimized selectivity for their target protein.
Streamlined scale-up
Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools. making purification scale-up straightforward.
Successful use – research scale
IMAC Sepharose 6 Fast Flow is trusted for protein purification in a wide body of research:
- Purification of native and mutant his-tagged T7 RNA polymerase
- Determination of sites on S. gordonii platelet adherence protein A (PadA) that interact with platelet GPIIb/IIIa
- Analysis of the metal-binding ability of a bZIP protein in Arabidopsis thaliana
Compatible empty columns – research scale
IMAC Sepharose 6 Fast Flow can be packed into gravity, Tricorn, HiScale, and XK columns.
Compatible empty columns – bioprocess scale
For process-scale applications, Cytiva recommends AxiChrom columns. IMAC Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.
Convenient prepacked columns – research scale
This IMAC protein purification resin is available in a range of different bulk pack sizes and in the following convenient prepacked formats:
- HiTrap IMAC FF for convenient use with a syringe, a pump or a chromatography systemHiPrep
- IMAC FF 16/10 for convenient scale-upHiScreen IMAC FF for method and process development
Prepacked IMAC Sepharose Fast Flow columns – bioprocess scale
Prepacked ReadyToProcess columns for bioprocess applications are available on request, eliminating the need for column packing and cleaning validation.
