Resin is composed of cross-linked 6% agarose beads modified with a chelating ligand, which is immobilized to the base matrix. The highly crosslinked resin enables high flow rates and productivity. Resin is supplied uncharged, allowing the user to charge with the metal ion that has optimized selectivity for their target protein.

Streamlined scale-up

Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools. making purification scale-up straightforward.

Successful use – research scale

IMAC Sepharose 6 Fast Flow is trusted for protein purification in a wide body of research:

• Purification of native and mutant his-tagged T7 RNA polymerase
• Determination of sites on S. gordonii platelet adherence protein A (PadA) that interact with platelet GPIIb/IIIa
• Analysis of the metal-binding ability of a bZIP protein in Arabidopsis thaliana

Compatible empty columns – research scale

IMAC Sepharose 6 Fast Flow can be packed into gravity, Tricorn, HiScale, and XK columns.

Compatible empty columns – bioprocess scale

For process-scale applications, Cytiva recommends AxiChrom columns. IMAC Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.

Convenient prepacked columns – research scale

This IMAC protein purification resin is available in a range of different bulk pack sizes and in the following convenient prepacked formats:

• HiTrap IMAC FF for convenient use with a syringe, a pump or a chromatography systemHiPrep
• IMAC FF 16/10 for convenient scale-upHiScreen IMAC FF for method and process development

Prepacked IMAC Sepharose Fast Flow columns – bioprocess scale

Prepacked ReadyToProcess columns for bioprocess applications are available on request, eliminating the need for column packing and cleaning validation.