The strong interaction between streptavidin and biotin can be used to purify antigens. Biotinylated antibody-antigen complexes bind to Streptavidin Sepharose High Performance, from which the antigen can be eluted. Another option is to exploit the interaction between 2-iminobiotin and streptavidin, which is weaker.

Compatible empty columns

Cytiva offers a variety of Life Sciences solutions for chromatography, including empty columns for packing your own columns. Streptavidin Sepharose High Performance is compatible for use with HiScale, Tricorn and XK columns.

Convenient prepacked columns

Streptavidin Sepharose High Performance is available in the following prepacked formats:

• HiTrap Streptavidin HP columns: easy for use with a syringe, pump, or chromatography system.
• Streptavidin HP SpinTrap: spin columns for use with a benchtop centrifuge
• Streptavidin HP MultiTrap: 96-well plates for high throughput screening.
• Streptavidin Mag Sepharose magnetic beads

Successful use in published research

Streptavidin Sepharose High Performance has been used in a variety of research applications, including:

• Study of voltage-gated sodium channels
• Purification of cell surface proteins