2’5’ ADP Sepharose 4B resin with immobilized NADP structural analog is primarily used for purification of enzymes requiring NADP as a cofactor.
- 2'5' ADP is an NADP structural analog immobilized on Sepharose 4B. It binds and immobilizes enzymes requiring this cofactor
2’5’ ADP Sepharose 4B interacts strongly with NADP+-dependent dehydrogenases. Selective elution with gradients of NAD+ or NADP+ has allowed the resolution of complex mixtures of dehydrogenase isoenzymes using 2’5’ ADP Sepharose 4B.
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