Purification of recombinant proteins is done under physiological conditions, which together with mild elution by maltose, preserves the activity of the target protein.

Dextrin Sepharose High Performance MBP resin

The small, evenly sized beads ensure that tagged proteins elute in narrow peaks, thus minimizing the need for further concentration steps. The resin is easily regenerated using 0.5 M NaOH, allowing the same column to be used to purify different proteins.

Successful use in published research

Research and applications using MBP resin include:

• Purifying tagged Arabidopsis Deg2 proteins to examine their crystal structure
• Purifying recombinant proteins to study RNA editing in Arabidopsis thaliana mitochondria

Compatible empty columns

Cytiva's solutions include a variety of empty columns. Dextrin Sepharose High Performance resin is compatible with Tricorn, XK, and HiScale column lines.

Convenient prepacked columns

This resin is also available as prepacked MBP-Trap columns. These HiTrap format columns can be used with a syringe, a pump, or a chromatography system.

Other Cytiva products for affinity purification

Looking for a different affinity resin? Let Cytiva help you find the right product to purify your tagged recombinant protein.