Enjoy Free Shipping on most orders placed online – Terms Apply

Ni Sepharose 6 Fast Flow histidine-tagged protein purification resin

Ni Sepharose 6 Fast Flow is a high binding-capacity IMAC chromatography resin for purifying his-tagged proteins. Good choice for purification at a variety of scales.

Order Product

ID	Product Name
17531804	Ni Sepharose 6 Fast Flow, 1 L
17531802	Ni Sepharose 6 Fast Flow, 100 mL
17531801	Ni Sepharose 6 Fast Flow, 25 mL
17531805	Ni Sepharose 6 Fast Flow, 5 L
17531806	Ni Sepharose 6 Fast Flow, 5 mL
17531803	Ni Sepharose 6 Fast Flow, 500 mL

Ni Sepharose 6 Fast Flow is a high binding-capacity IMAC chromatography resin for purifying his-tagged proteins. Good choice for purification at a variety of scales.

In process scale applications: This resin is used to purify histidine-tagged (his-tagged) and untagged recombinant proteins even at large scales.
In research scale applications: This resin binds 40 mg his-tag protein/mL resin and is suitable for use in batch mode, with gravity flow, or when scalability is needed.
Meets industrial demands with security of supply, robust performance, and regulatory support.
Available as bulk resin and as prepacked columns for use with manual methods or a chromatography system.
Excellent flow properties provide great performance even at high flow rates.

High binding capacity resin with excellent flow properties

Ni Sepharose 6 Fast Flow (FF) is composed of cross-linked 6% agarose beads modified with a chelating ligand immobilized to the base matrix and precharged with nickel ions.

The high dynamic binding capacity combined with the ability to withstand high flow rates gives an overall advantage of being able to increase flow rate and reduce the total time for protein purification considerably with a minimal loss in recovery. Therefore, this immobilized metal affinity chromatography (IMAC) purification resin is a good choice for scaling up.

Successful use in research-scale applications

Life science professionals use Ni Sepharose 6 Fast Flow for his-tag protein purification:

Customer feedback on use in batch purification
IMAC chromatography of human β2-microglobulin in E. coli to 95% purity
Scale-up purification of a viral capsid protein from a prepacked column

Compatibile empty columns – research scale

Cytiva offers a variety of empty columns for packing your own columns. Ni Sepharose 6 Fast Flow is compatible for use with empty PD gravity flow, HiScale, Tricorn, and XK columns.

Compatible empty columns – bioprocess scale

For process-scale applications, Cytiva recommends AxiChrom columns. Ni Sepharose 6 Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.

Prepacked formats – research scale

Ni Sepharose 6 Fast Flow resin is also available in the following prepacked formats:

HisGraviTrap gravity flow column: for simple purification without the need for equipment
His MultiTrap FF 96-well plate: for high throughput screening and small scale purification using centrifuge or vacuum equipment
HisTrap FF crude: HiTrap column format for convenient use with a syringe, a peristaltic pump or a chromatography system.
HiScreen Ni FF column for method and process development
HisPrep FF column for convenient scale-up

Prepacked formats – bioprocess scale

Prepacked ReadytoProcess columns for bioprocess applications are available on request, eliminating the need for column packing and cleaning validation.

Other IMAC chromatography resins and columns

Life Sciences solutions from Cytiva include a variety of nickel-charged resins and other IMAC products. Options for high purity, high resolution, direct loading of cell culture supernatants, and loading of unclarified E. coli lysates are available.

Read less

Select your model

Parameter	{{main.activeVariantName}}
There's no available product features to display for this product variant.
^{{{main.calculateFootnoteIndex(feature, main.features.ownFeatures)}}}

^{{{main.calculateFootnoteIndex(feature, main.features.ownFeatures)}}}{{feature.footnote}}

Parameter	{{referenceSection.referenceName}}
There's no available product features to display for this product variant.
^{{{main.calculateFootnoteIndex(feature, referenceSection.featureValues)}}}

^{{{main.calculateFootnoteIndex(feature, referenceSection.featureValues)}}}{{feature.footnote}}

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.

Product	Product Name	Pack size All 5 mL 1 L 100 mL 25 mL 5 L 500 mL	Price
17531801	Ni Sepharose 6 Fast Flow, 25 mL	25 mL	311.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
17531802	Ni Sepharose 6 Fast Flow, 100 mL	100 mL	1 041.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
17531803	Ni Sepharose 6 Fast Flow, 500 mL	500 mL	4 480.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
17531804	Ni Sepharose 6 Fast Flow, 1 L	1 L	On Request	Get Quote
17531805	Ni Sepharose 6 Fast Flow, 5 L	5 L	On Request	Get Quote
17531806	Ni Sepharose 6 Fast Flow, 5 mL	5 mL	77.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock

Ni Sepharose 6 Fast Flow histidine-tagged protein purification resin

Ni Sepharose 6 Fast Flow histidine-tagged protein purification resin

High binding capacity resin with excellent flow properties

Successful use in research-scale applications

Compatibile empty columns – research scale

Compatible empty columns – bioprocess scale

Prepacked formats – research scale

Prepacked formats – bioprocess scale

Other IMAC chromatography resins and columns

Refine

Customers also bought

Leave a Review

Product Support

Looking for support assistance?

Ni Sepharose 6 Fast Flow histidine-tagged protein purification resin

Ni Sepharose 6 Fast Flow histidine-tagged protein purification resin

High binding capacity resin with excellent flow properties

Successful use in research-scale applications

Compatibile empty columns – research scale

Compatible empty columns – bioprocess scale

Prepacked formats – research scale

Prepacked formats – bioprocess scale

Other IMAC chromatography resins and columns

Select your model

{{referenceSection.sectionTitle}}

Refine

Customers also bought

Leave a Review

Product Support

Looking for support assistance?