The high dynamic binding capacity combined with the ability to withstand high flow rates gives an overall advantage of being able to increase flow rate and reduce the total time for protein purification considerably with a minimal loss in recovery. Therefore, this immobilized metal affinity chromatography (IMAC) purification resin is a good choice for scaling up.

Successful use in research-scale applications

Life science professionals use Ni Sepharose 6 Fast Flow for his-tag protein purification:

• Customer feedback on use in batch purification
• IMAC chromatography of human β2-microglobulin in E. coli to 95% purity
• Scale-up purification of a viral capsid protein from a prepacked column

Compatibile empty columns – research scale

Cytiva offers a variety of empty columns for packing your own columns. Ni Sepharose 6 Fast Flow is compatible for use with empty PD gravity flow, HiScale, Tricorn, and XK columns.

Compatible empty columns – bioprocess scale

For process-scale applications, Cytiva recommends AxiChrom columns. Ni Sepharose 6 Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.

Prepacked formats – research scale

Ni Sepharose 6 Fast Flow resin is also available in the following prepacked formats:

• HisGraviTrap gravity flow column: for simple purification without the need for equipment
• His MultiTrap FF 96-well plate: for high throughput screening and small scale purification using centrifuge or vacuum equipment
• HisTrap FF crude: HiTrap column format for convenient use with a syringe, a peristaltic pump or a chromatography system.
• HiScreen Ni FF column for method and process development
• HisPrep FF column for convenient scale-up

Prepacked formats – bioprocess scale

Prepacked ReadytoProcess columns for bioprocess applications are available on request, eliminating the need for column packing and cleaning validation.

Other IMAC chromatography resins and columns

Life Sciences solutions from Cytiva include a variety of nickel-charged resins and other IMAC products. Options for high purity, high resolution, direct loading of cell culture supernatants, and loading of unclarified E. coli lysates are available.