Ni Sepharose excel is a his-tagged protein purification resin for minimized Ni-leakage and maximized protein recovery when samples contain stripping agents.
- Versatile – suitable for eukaryotic samples, including insect and CHO cells, as well as bacterial samples such as E. coli.
- Direct sample load – of eukaryotic cell culture supernatants, eliminating the need for pretreatment of samples that usually cause nickel stripping.
- Retained binding capacity of up to 10 mg/mL resin.
- Simplified sample handling – fewer steps needed compared with conventional workflow.
- Flexible formats – prepacked and loose resin formats for optimized screening and preparative his-tag purification.
EDTA-resistant resin for IMAC chromatography
Ni Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a chelating ligand. Nickel ions remain bound to the ligand even after 24 h incubation in 10 mM EDTA.
Read moreProduct | Product Name | Price | ||
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17371201 | Ni Sepharose excel, 25 mL | 25 mL | 434.28 USD |
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In Stock Stock on request Limited stock |
17371202 | Ni Sepharose excel, 100 mL | 100 mL | 1 664.38 USD |
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In Stock Stock on request Limited stock |
17371203 | Ni Sepharose excel, 500 mL | 500 mL | 6 615.17 USD |
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In Stock Stock on request Limited stock |
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