Ni Sepharose excel is a his-tagged protein purification resin for minimized Ni-leakage and maximized protein recovery when samples contain stripping agents.

  • Versatile – suitable for eukaryotic samples, including insect and CHO cells, as well as bacterial samples such as E. coli.
  • Direct sample load – of eukaryotic cell culture supernatants, eliminating the need for pretreatment of samples that usually cause nickel stripping.
  • Retained binding capacity of up to 10 mg/mL resin.
  • Simplified sample handling – fewer steps needed compared with conventional workflow.
  • Flexible formats – prepacked and loose resin formats for optimized screening and preparative his-tag purification.

EDTA-resistant resin for IMAC chromatography

Ni Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a chelating ligand. Nickel ions remain bound to the ligand even after 24 h incubation in 10 mM EDTA.

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Product Product Name Price
17371201 Ni Sepharose excel, 25 mL 25 mL 434.28 USD Add to cart

In Stock Stock on request Limited stock

17371202 Ni Sepharose excel, 100 mL 100 mL 1 664.38 USD Add to cart

In Stock Stock on request Limited stock

17371203 Ni Sepharose excel, 500 mL 500 mL 6 615.17 USD Add to cart

In Stock Stock on request Limited stock

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