Because of this strong binding, samples that typically cause stripping of metal ions can be loaded directly onto the resin without time-consuming pretreatment. Therefore, this his tag purification resin is especially suitable for purifying histidine-tagged (his-tagged) proteins secreted into eukaryotic cell culture supernatants.

His tag purification of large sample volumes

The flow properties of Ni Sepharose excel nickel resin make it well-suited to purification at a wide range of scales. This resin can handle loading of large sample volumes, so you can purify low concentrations of target proteins using manual or automated methods.

Successful use in published research

Scientists use Ni Sepharose excel to purify his-tagged recombinant proteins from eukaryotic and prokaryotic samples, as cited in these publications:

• Human transferrin receptor 1 secreted from BHK cells
• Proprotein convertase subtilisin/kexin type 9 from HEK293 supernatants
• Membrane protein insertase EcYidC from E. coli for crystallization
• Helicobacter pylori adhesin BabA from periplasmic extracts

Convenient prepacked columns

Ni Sepharose excel resin is also available in the following formats:

• HisTrap excel column: HiTrap format for convenient use with a syringe, a peristaltic pump, or a chromatography system.
• His MagSepharose excel: Magnetic beads for small-scale purification and screening. They are appropriate for early stages of research when many parallel experiments are required.