This IMAC resin has a binding capacity that exceeds 40 mg/mL, resulting in high protein recovery. The small bead size contributes to a more efficient purification compared to nickel resins of larger bead size.

Successful use in published research

Applications of this affinity chromatography resin include:

• Purification of histidine-tagged proteins to study how transcription regulators oligomerize
• Isolation of recombinant HIV-1 protease from inclusion bodies in a tandem procedure with Q Sepharose Fast Flow

Compatible empty columns

Cytiva offers a variety of empty columns for packing your own columns. Ni Sepharose High Performance is compatible for use with empty HiScale, Tricorn, and XK columns.

Convenient prepacked columns

Ni Sepharose High Performance resin is available in the following prepacked formats:

• HisTrap HP column: HiTrap format (1 mL or 5 mL) for convenient use with a syringe, a peristaltic pump, or a chromatography system.
• His SpinTrap: Spin columns for screening of small sample quantities using a benchtop centrifuge.
• His MultiTrap HP: 96-well plate for high throughput screening and small scale purification using centrifuge or vacuum equipment.
• His Mag Sepharose Ni magnetic beads: designed for small-scale purification and screening.

Other IMAC affinity chromatography resins and columns

Cytiva's solutions include a variety of nickel-charged resins and other IMAC products. Options for high purity, high resolution, direct loading of cell culture supernatants, loading of unclarified E. coli lysates, and charging with your metal ion of choice are available.