The resin’s small bead size (34 μm) allows elution in narrow peaks. Purification and elution conditions are designed to be mild and mimic physiological conditions, preserving the target protein’s activity.

This robust and stable Sepharose-based resin yields reproducible purifications and minimizes the need for further concentration steps. See our StrepTactin Sepharose application note for an example of a single-step purification that achieved greater than 95% purity.

Successful use in published research

StrepTactin Sepharose is used for tagged protein purification in a variety of applications, including:

• Study of the formation and structure of a NAIP5-NLRC4 inflammasome
• Analysis of how a staphylococcal protein evades toll-like receptor activation

Compatible empty columns

Cytiva solutions include a variety of empty columns for packing your own columns. StrepTactin Sepharose is compatible for use with Tricorn and XK columns.

Convenient prepacked columns

StrepTactin Sepharose High Performance is also available in a prepacked format:

• StrepTrap: HiTrap format for convient use with a syringe, a pump, or a chromatography system.