His-tagged proteins sometimes require cobalt-charged IMAC resin when nickel is not the optimal choice of metal ion. TALON Superflow is a high-quality option that offers different selectivity for affinity chromatography, typically providing protein with higher purity compared with nickel-based resins.

Successful use in published research

Applications for this his tag purification resin include:

• Purification of his-tagged antibody fragments to study Bothrops jararacussu venommyotoxicity

Convenient prepacked formats

TALON Superflow resin is available in the following prepacked formats:

• HiTrap TALON crude: HiTrap column format for convenient use with a syringe, a peristaltic pump, or a chromatography system
• His SpinTrap TALON: Spin columns for screening of small sample quantities using a benchtop centrifuge
• His GraviTrap TALON: gravity flow column for simple purification without the need for equipment
• His MultiTrap TALON 96-well plate: for high throughput screening and small scale purification using centrifuge or vacuum equipment