Overview of chromatography
Chromatography is a separation technique that helps separate molecules from complex mixtures. Liquid chromatography helps separate biomolecules in a liquid mobile phase using solid stationary phases. This chromatography method supports use in preparative or analytical applications. In column liquid chromatography, the liquid phase moves through a chromatography column that contains a stationary phase. As the sample moves over the column, biomolecules in the mixture are retained by the column matrix or pass through depending on their physico-chemical properties.
Common types of liquid chromatography include:
- Size exclusion chromatography
- Ion exchange chromatography
- Hydrophobic interaction chromatography
- Affinity chromatography
- Mixed-mode or multimodal chromatography
Liquid chromatography system components
Liquid chromatography systems comprise an array of components that work to move sample solutions and buffers through the system and collect fractions containing the molecule of interest as well as liquid waste. Pumps move liquids through tubing, which connects to valves, columns, monitors that measure key sample characteristics, fraction collectors, and other components. Automated systems are also connected to a computer that runs software to control the chromatography process and collect sample and run data.
Chromatography columns are key components of a liquid chromatography system. They contain the stationary phase, often a resin, that helps isolate constituent parts from a sample. We offer prepacked columns as well as bulk resins and empty columns for various types of chromatography at a range of scales, from lab scale to process development to manufacturing. Chromatography software helps maintain peak operation parameters in your system for peak results.
Liquid chromatography pumps
Chromatography pumps are devices that push the mobile phase through a chromatography system.
Types of chromatography pumps
We offer metered piston sample pumps, which pump sample into the system at a specified volume, as well as peristaltic pumps for our chromatography systems. Peristaltic pumps use a rotor to pinch tubing and generate a vacuum that moves liquid through the chromatography system.
Chromatography pumps FAQs
Below are answers to some frequently asked questions about chromatography pumps.
What is the role of a pump in chromatography?
The pumps used in liquid chromatography help force the mobile phase, buffers, and solutions through the system. Most liquid chromatography systems rely on pumps for this purpose.
What type of pump does my ÄKTA™ chromatography system use?
Use our Purify web app to find out what components you need for your ÄKTA™ system. Select your system from the dropdown menu to see a list of recommended parts and accessories.