pEZZ 18 Protein A Gene Fusion Vector

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For rapid expression of secreted fusion proteins and their one-step purification using IgG Sepharose 6 Fast Flow

The phagemid pEZZ 18 contains the protein A signal sequence and two synthetic “Z” domains based on the “B” IgG binding domain of protein A. Proteins are expressed as fusions with the “ZZ” peptide and secreted into the aqueous culture medium under the direction of the protein A signal sequence. They are easily purified using IgG Sepharose 6 Fast Flow to which the “ZZ” domain binds tightly. Because of its folding properties, the 14 kDa “ZZ” peptide has little effect on folding of the fusion partner into a native conformation.

Expression

Expression is controlled by the lacUV5 and protein A promoters and is not inducible. Elements of the protein A gene provide the ATG and ribosome-binding sites. Stop codons must be provided by the insert

Sequencing

M13 Universal Sequencing Primer can be used for both double-stranded and single-stranded sequencing. A protocol for production of single-stranded DNA is provided with the vector

Cloning

Inserts containing a stop codon will yield white colonies when grown on media containing X-gal

Host(s)

E. coli strains carrying a lac deletion but capable of a-complementation of lacZ’.

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Product	Product Name	Price
27481001	pEZZ 18 Protein A Gene Fusion Vector	Discontinued

pEZZ 18 Protein A Gene Fusion Vector

Expression

Sequencing

Cloning

Host(s)

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