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PlasmidPrep Mini Spin Kit

Purify high yields of plasmid DNA in less than 10 min. Simplify the protocol and purified DNA is suitable for downstream applications.

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PlasmidPrep Mini Spin Kit uses a simple plasmid DNA purification protocol involving a modified alkaline lysis procedure and a novel silica-based membrane to achieve highly efficient plasmid DNA purification.

Produces high-quality plasmid DNA with excellent reproducibility for use in cloning, restriction enzyme digestion, PCR amplification, and DNA sequencing
Total preparation time of less than 10 min represents a 50% reduction in protocol time
Increased ease of use provided by minimal changes in pipetting volume from one part of the protocol to the next
Fewer centrifugation steps
Color-coded tubes and columns simplify the protocol
No organic solvents such as phenol chloroform

The extracted plasmid DNA resulting from performing a miniprep is called a "plasmid miniprep" and is often performed using a plasmid purification kit. Minipreps are used in the process of molecular cloning to analyze bacterial clones. When bacteria are lysed under alkaline conditions, both chromosomal DNA and protein are denatured, but the plasmid DNA remains stable. On addition of acetate-containing neutralization buffer the larger and less supercoiled genomic DNA and proteins precipitate, but the small bacterial DNA plasmids stay in the solution.

The typical plasmid DNA yield from a fresh 1.5 mL culture of E. coli containing a high copy number plasmid is approximately 9 μg. Instead of organic solvents, the kit contains chaotropic salts to denature protein components and promote selective binding of DNA to the novel silica membrane.

The kit contains spin columns prepacked with a novel silica membrane, suspension, lysis, and neutralization buffers containing chaotropic salts, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.

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Product	Product Name	Pack size All 10 50 250	Price
28904269	Amersham plasmidPrep Mini Spin Kit	50	123.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28904270	Amersham plasmidPrep Mini Spin Kit	250	392.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28904271	illustra Plasmid Mini Spin	10	On Request	Get Quote

PlasmidPrep Mini Spin Kit

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