CyDye Post-Labeling Reactive Dye Packs
CyDye Post-Labeling Reactive Dye Packs
CyDye Post-Labeling Reactive Dye Packs are optimized for microarray probe labeling using reactive Cy3 and Cy5 monofunctional NHS ester.
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CyDye Post-Labeling Reactive Dye Pack for the generation of highly fluorescent Cy3- and Cy5-labeled probes via post-labeling (amino allyl) method.
- Uses Reactive CyDye fluorescent dyes: optimized for microarray probe labeling
- Provides conveniently packaged, highly reactive Cy3 and Cy5 monofunctional NHS ester: to label probes via the post-labeling (amino allyl) route for use in microarray applications
A two-step protocol to prepare cDNA probes labeled with CyDye fluorescent dyes
In the first step, reverse transcriptase synthesizes first-strand cDNA, incorporating a chemically reactive nucleotide analog (amino allyl-dUTP) into the cDNA. The mRNA template is then degraded and the cDNA purified to remove free nucleotides and primers. In the second step, the purified cDNA is post-labeled with the reactive forms of Cy3 or Cy5 NHS esters that bind to the modified amino allyl-nucleotides. After a final purification, the labeled cDNA is ready for hybridization.
| Product | Product Name | Price | ||
|---|---|---|---|---|
| RPN5661 |
CyDye Post-Labeling Reactive Dye Pack
|
12 x Cy3 12 x Cy5 | 796.00 USD |
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| 25801080 |
Cy5 Post-Labeling Reactive Dye Pack
|
Discontinued | ||
| 25801079 |
Cy3 Post-Labeling Reactive Dye Pack
|
Discontinued |
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