Thermo Sequenase DNA polymerase is a novel thermostable DNA polymerase that uses ddNTPs as readily as dNTP substrates at temperatures similar to those used in standard PCR. The result is:

  • Uniform and easy-to-read band patterns
  • Better incorporation of ddNTPs helps avoid secondary structures that can cause unreadable sequences
  • Read-through of difficult sequences (e.g., GC-rich), due to the ability of thermostable enzymes to function at elevated temperature used for sequencing

ddNTPs are chain-elongating inhibitors of DNA polymerase, which are used in the Sanger method for DNA sequencing. Each nucleotide base of that particular type has a probability of being bonded not to a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation. This property, in addition to thermostability, makes Thermo Sequenase DNA polymerase an excellent choice for cycle sequencing where template quantity limitations preclude the use of T7 Sequenase DNA polymerase.

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Product Product Name Price
E79000Y Thermo Sequenase DNA Polymerase (with Thermoplasma acidophilum Inorganic Pyrophosphatase (TAP)) 1000 units 935.00 USD Add to cart Get Quote

In Stock Stock on request Limited stock

E79000Z Thermo Sequenase DNA Polymerase (with Thermoplasma acidophilum Inorganic Pyrophosphatase (TAP)) 10000 units 6 074.00 USD Add to cart Get Quote

In Stock Stock on request Limited stock

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