Thermo Sequenase DNA Polymerase

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Thermo Sequenase DNA polymerase is a novel thermostable DNA polymerase that uses ddNTPs as readily as dNTP substrates at temperatures similar to those used in standard PCR. The result is:

Uniform and easy-to-read band patterns
Better incorporation of ddNTPs helps avoid secondary structures that can cause unreadable sequences
Read-through of difficult sequences (e.g., GC-rich), due to the ability of thermostable enzymes to function at elevated temperature used for sequencing

ddNTPs are chain-elongating inhibitors of DNA polymerase, which are used in the Sanger method for DNA sequencing. Each nucleotide base of that particular type has a probability of being bonded not to a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation. This property, in addition to thermostability, makes Thermo Sequenase DNA polymerase an excellent choice for cycle sequencing where template quantity limitations preclude the use of T7 Sequenase DNA polymerase.

The properties of Thermo Sequenase also enable its use in many SNP genotyping, genotyping by sequencing, or genome sequencing applications where primer extension is employed.

DNA polymerases such as Thermo Sequenase can be incorporated into custom-made cycle sequencing kits, using our custom manufacturing facilities.

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Product	Product Name	Pack size All 1000 units 10000 units	Price
E79000Z	Thermo Sequenase DNA Polymerase (with Thermoplasma acidophilum Inorganic Pyrophosphatase (TAP))	10000 units	6 194.26 USD	Add to cart Get Quote In Stock Stock on request Limited stock
E79000Y	Thermo Sequenase DNA Polymerase (with Thermoplasma acidophilum Inorganic Pyrophosphatase (TAP))	1000 units	953.51 USD	Add to cart Get Quote In Stock Stock on request Limited stock

Thermo Sequenase DNA Polymerase

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