Exonuclease I is used to selectively digest excess single stranded DNA in a mixture also containing double-stranded DNA, such as removing unused primers after PCR.

  • 3'5' single-stranded exonuclease.
  • Monomeric 54.5 kDa bacterial exonuclease I recombinantly expressed in E. coli.
  • Inactivated by heating at 80°C for 15 minutes.
  • Active in PCR buffers.

Exonuclease I, used together with Alkaline Phosphatase for inactivation of dNTPs, provides effective cleanup of PCR reactions for subsequent use in DNA sequencing.

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Product Product Name Price
E70073X Exonuclease I 5000 units 800.00 USD Add to cart


E70073Z Exonuclease I 2500 units 470.00 USD Add to cart


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