Exonuclease I
Exonuclease I
Exonuclease I catalyzes sequential removal of nucleotides from the 3' end of single-stranded DNA molecules.
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Exonuclease I is used to selectively digest excess single stranded DNA in a mixture also containing double-stranded DNA, such as removing unused primers after PCR.
- 3'→5' single-stranded exonuclease.
- Monomeric 54.5 kDa bacterial exonuclease I recombinantly expressed in E. coli.
- Inactivated by heating at 80°C for 15 minutes.
- Active in PCR buffers.
Exonuclease I, used together with Alkaline Phosphatase for inactivation of dNTPs, provides effective cleanup of PCR reactions for subsequent use in DNA sequencing.
Read more| Product | Product Name | Price | ||
|---|---|---|---|---|
| E70073X | Exonuclease I | 5000 units | 800.00 USD |
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| E70073Z | Exonuclease I | 2500 units | 470.00 USD |
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