TempliPhi Large Construct Kit uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates specifically from large DNA vectors (e.g., fosmids and BACs).

  • Developed for high-throughput users and specifically designed for BAC or fosmid DNA preparation.
  • Start from microliter quantities of purified templates, as well as bacterial culture or glycerol stocks.
  • Generate 5 μg amounts of total DNA.
  • Simplified process, 20 min hands-on time.
  • Amplification is completed in 18 h with no need for thermal cycling.
  • Simple, automation-friendly protocol with great reproducibility.

Trends in rolling circle amplification: Capabilities for manipulation, error free production and assembly of nucleic acids are advancing. Find out how TempliPhi amplification kits use isothermal rolling circle amplification (RCA), enabled by phi29 DNA polymerase, to support these advancements.

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Product Product Name Price
25640080 TempliPhi Large Construct Kit 1000 reactions 3,952.00 USD Add to cart


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