The kit provides 50 preformulated, single-dose reaction beads prepackaged in thin-walled 0.5 ml tubes compatible with most thermal cyclers. For synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice.

• For synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice for RT-PCR.
• Predispensed, ambient-temperature-stable, single-dose reaction beads minimize pipetting errors, avoid cross-contamination, and ensure optimal performance.
• First-strand reaction beads contain no primer, thus allowing users to select a first-strand primer of choice.
• Highly suitable for research applications that use PCR to detect and quantify eukaryotic RNA from a variety of samples.
• Reaction beads are function-tested for first-strand synthesis of cDNA up to 7.5 kb and in RT-PCR from blood samples.
• Completed reactions (33 µl final volume) can be used directly in PCR after adding water, Taq DNA polymerase, and primers; first-strand cDNA can also be used as a template for traditional Gubler-Hoffman second-strand cDNA synthesis.