MicroSpin G-25 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, and removal of unincorporated nucleotides from end-labeled oligonucleotides.

  • Excellent for rapid buffer exchange/desalting: for example, of PCR amplification products and other DNAs in a volume of 10 to 100 µL using spin-column chromatography
  • Excellent for rapid DNA purification: especially newly synthesized oligonucleotides > 10-mers in 100 to 150 µL of deprotection solution
  • Convenient: Prepacked with Sephadex G-25 DNA Grade and pre-equilibrated in distilled wa-ter containing 0.05% Kathon CG/ICP Biocide
  • Ready to use: requires less than 4 minutes from sample application to collection of purified oligonucleotides
  • Tested in assays: including nickase, single- and double-stranded exonuclease, and RNase
  • Use additionally: for desalting/buffer exchange of DNA and removal of unincorporated radi-onucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 µL

MicroSpin G-25 columns for rapid DNA purification in desalting and buffer exchange applications

MicroSpin G-25 columns are appropriate for use with DNA greater than 10 bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or labeling reactions. They will not remove or denature enzymes.

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Product Product Name Price
27532501 Amersham MicroSpin G-25 Columns 50 columns 285.00 USD Add to cart


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