NICK Columns are used for the purification of nick-translated DNA fragments and for separation of any labeled probe from unincorporated labeled nucleotides.

  • For rapid purification of labeled DNA (≥ 20 bases in length) from unincorporated radiolabeled nucleotides using gravity-flow chromatography
  • Prepacked with Sephadex G-50 DNA Grade (see Sephadex G-50 DNA Grade and Sephadex G-100 DNA Grade) in distilled water with 0.15% Kathon CG/ICP Biocide
  • Can accommodate sample volumes up to 100 μl

NICK columns are ready for immediate use and operate by gravity flow. Tests show that at least 97% of radioactivity applied in a nick-translated mixture elutes in two well-separated peaks, corresponding to labeled DNA molecules and unincorporated nucleotides, respectively. At least 90% of the applied DNA is recovered and can then be used in DNA hybridization protocols. Maximum sample volume is 100 μL.

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Product Product Name Price
17085501 Amersham NICK Columns 20 columns 212.00 USD Add to cart Get Quote

In Stock Stock on request Limited stock

17085502 Amersham NICK Columns 50 columns 465.00 USD Add to cart Get Quote

In Stock Stock on request Limited stock

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