NICK Columns are used for the purification of nick-translated DNA fragments and for separation of any labeled probe from unincorporated labeled nucleotides.

  • For rapid purification of labeled DNA (≥ 20 bases in length) from unincorporated radiolabeled nucleotides using gravity-flow chromatography
  • Prepacked with Sephadex G-50 DNA Grade (see Sephadex G-50 DNA Grade and Sephadex G-100 DNA Grade) in distilled water with 0.15% Kathon CG/ICP Biocide
  • Can accommodate sample volumes up to 100 μl

NICK columns are ready for immediate use and operate by gravity flow. Tests show that at least 97% of radioactivity applied in a nick-translated mixture elutes in two well-separated peaks, corresponding to labeled DNA molecules and unincorporated nucleotides, respectively. At least 90% of the applied DNA is recovered and can then be used in DNA hybridization protocols. Maximum sample volume is 100 μL.

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Product Product Name Price
17085501 Amersham NICK Columns 20 columns 257.00 USD Add to cart


17085502 Amersham NICK Columns 50 columns 564.00 USD Add to cart


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