Designed for the rapid purification of DNA, in conjunction with Sephadex G-25 DNA Grade or Sephadex G-50 DNA Grade, for desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.

  • For rapid buffer exchange/desalting of PCR products and other DNA samples in a volume of 10 to 100 µL using spin-column chromatography and Sephadex G-25 DNA Grade (not included)
  • Excellent for rapid purification of newly synthesized oligonucleotides > 10-mers in 100 to 150 µL of deprotection solution using spin-column chromatography
  • Flexible for your experiments, allowing differing amounts of Sephadex
  • Can also be used for desalting/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 µL

Sephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and with a spin protocol this means it can be used for any DNA greater than 10 bases in length.

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Product Product Name Price
27356501 MicroSpin Columns, 50 Pieces 160.96 USD Add to cart Get Quote

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