Convenient and efficient mRNA isolation

Eukaryotic mRNA makes up only 1 to 3% of total cellular RNA, so efficient mRNA purification is critical. Sera-Mag Oligo(dT) Magnetic Particles remove 90% or more of mRNA from total RNA with just one extraction. Sera-Mag Oligo(dT) Magnetic Particles bind target mRNA through pairing of the polyadenylated RNA tail found on the 3’ end of mRNA to the covalently bound oligo(dT) groups on the surface of the particles. This binding is easily accomplished using standard hybridization conditions during the mRNA purification process.

Fast magnetic response times and stable physical integrity

The core of the particle is made by a free radical emulsion polymerization of styrene and acid monomer. One layer of magnetite is coated onto this core, while the surface is modified to minimize non-specific binding of proteins. Oligo(dT) groups are then covalently bound to the particle surface. Since the magnetite is encapsulated, there is no exposed iron minimizing interference with downstream enzymatic applications.

Customized magnetic bead solutions that fit seamlessly into your workflows can be developed for specific applications, often saving you time and money. This product can be provided ready-to-use with little or no need for further modification allowing you to focus on your research. To learn more, please visit our custom manufacturing services page.