Designed to select for small-fragment cfDNA while minimizing genomic DNA contamination, Sera-Xtracta™ Cell-Free DNA Kit* offers reliable extraction and purification of cfDNA for liquid biopsy from samples including plasma, serum and urine. High yield and sensitivity make the kit well-suited for applications such as cancer diagnosis and monitoring, even when sample is limited.

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Read application note: Detection of cancer-associated mutations in plasma from stage IV Non-small cell lung cancer (NSCLC) patient samples

Read application note: Targeted NGS biomarker discovery using serum from stage IV non-small cell lung cancer (NSCLC) and colorectal cancer (CLC) patient samples

Read application note: Plasma based-monitoring of therapeutic response and resistance in patients positive for epidermal growth factor receptor (EGFR) biomarker

  • Efficient recovery of small-fragment cfDNA in the size range 50-300 bp: capture more cfDNA to increase sensitivity levels.
  • Minimal co-purification of higher molecular weight genomic DNA due to active selection of smaller fragments which improves sensitivity in downstream applications.
  • Scalability: one kit to cover up to 4 mL input of sample.
  • Efficient extraction: procedure can be completed in 120 minutes.
  • Ease of use: compatible with molecular biology techniques, including next-generation sequencing (NGS), qPCR, ddPCR, BEAMing and other amplification and genotyping applications.

The method uses chaotropic agents and detergents to disrupt protein components to which the cfDNA may initilally bind and to promote the selective binding of this cfDNA to the silica magnetic beads.

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Product Product Name
Price
29437807 Sera-Xtracta™ Cell-Free DNA Kit 96 purifications 908.00 USD Add to cart

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