Purification to ensure species-specificity

The antibodies are prepared by hyper-immunizing an animal with purified immunoglobulin fractions from normal human serum to produce high affinity antibodies. The pooled antiserum is used to produce an immunoglobulin preparation that is then affinity adsorbed to remove cross-reacting antibodies with the immunoglobulins of other species. These activities are thoroughly depleted to ensure species-specificity. Finally, to select for specific binding to human IgG, the antibodies are purified using an affinity column of human IgG. After washing to remove non-specific serum components and low affinity antibodies, the species-specific antibodies are eluted using carefully selected, mild conditions that minimize aggregation and preserve immunological activity, yet which will elute high affinity antibodies.