Hybond N+ is a positively charged nylon membrane capable of high sensitivity in DNA and RNA blotting.

  • Positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm² recommended for use with radioactive or nonradioactive chemiluminescence and chemifluorescence detection systems.
  • Recommended for all high density membrane arrays.
  • Yields excellent sensitivity in conventional Southern blotting.

Nucleic acid samples may be fixed by UV exposure or baking, although UV fixation gives better reproducibility. Hybond-N+ is not suitable for alkali blotting and fixation. All Hybond membranes are manufactured specifically for life science applications. Production runs are carefully controlled and the product exhaustively screened to ensure that only the most consistent product reaches the user. All Hybond membranes are identical on both sides. Supported nylon membranes have a high binding capacity for nucleic acid, in addition to high tensile strength. For applications requiring a high degree of sensitivity and/or reprobing these types of membranes are an ideal choice. Due to its high protein binding capacity, nylon requires extensive blocking prior to detection with antibodies to avoid high backgrounds. Consequently, nylon membranes are not recommended for use in Western blotting.

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Product Product Name Price
RPN87B Hybond-N+ (87 mm) Ø 87 mm Discs Discontinued
RPN3050B Hybond-N+ (30 × 50 cm) Sheets Discontinued
RPN119B Hybond-N+ (11.9 × 7.8 cm) 11.9 x 7.8 cm Sheets 50 487.00 USD Add to cart

In Stock Stock on request Limited stock

RPN1210B Hybond-N+ (12 × 10 cm) 12 x 10 cm Sheets 20 364.00 USD Add to cart

In Stock Stock on request Limited stock

RPN1520B Hybond-N+ (15 × 20 cm) 15 x 20 cm Sheets 10 479.00 USD Add to cart

In Stock Stock on request Limited stock

RPN1510B Hybond-N+ (15 × 10 cm) 15 x 10 cm Sheets 20 364.00 USD Add to cart

In Stock Stock on request Limited stock

RPN82B Hybond-N+ (82 mm) Ø 82 mm Discs 50 342.00 USD Add to cart

In Stock Stock on request Limited stock

RPN303B Hybond-N+ (30 cm × 3 m) 30 cm x 3 m Roll 1 443.00 USD Add to cart

In Stock Stock on request Limited stock

RPN2250B Hybond-N+ (22.2 × 22.2 cm) 22.2 x 22.2 cm Sheets Discontinued
RPN203B Hybond-N+ (20 cm × 3 m) 20 cm x 3 m Roll 1 428.00 USD Add to cart

In Stock Stock on request Limited stock

RPN2222B Hybond-N+ (22.2 × 22.2 cm) 22.2 x 22.2 cm Sheets 10 428.00 USD Add to cart

In Stock Stock on request Limited stock

RPN2020B Hybond-N+ (20 × 20 cm) 20 x 20 cm Sheets 10 415.00 USD Add to cart

In Stock Stock on request Limited stock

RPN1782B Hybond-N+ (82 mm) Ø 82 mm Gridded Discs Discontinued

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