Amersham Hybond-NX is a neutral nylon membrane with all the properties of Hybond-N. It can be used for multiple re-probing due to its high tensile strength. Hybond-NX gives cleaner backgrounds than Hybond-N when using hybridization buffer volumes below 70 µL/cm² of membrane.

  • Volumes of at least 30 µL hybridization buffer per cm² of membrane should be used with this membrane.

All Hybond membranes are manufactured specifically for life science applications. Production runs are carefully controlled and the product exhaustively screened to ensure that only the most consistent product reaches the user. All Hybond membranes are identical on both sides. Supported nylon membranes have a high binding capacity for nucleic acid, in addition to high tensile strength. For applications requiring a high degree of sensitivity and/or reprobing these types of membranes are an ideal choice. Due to its high protein binding capacity, nylon requires extensive blocking prior to detection with antibodies to avoid high backgrounds. Consequently, nylon membranes are not recommended for use in Western blotting.

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Product Product Name Price
RPN303T Hybond-NX (30 cm × 3 m) 30 cm x 3 m 1 roll 403.00 USD Add to cart

In Stock Stock on request Limited stock

RPN203T Hybond-NX (20 cm × 3 m) 20 cm x 3 m 1 roll 398.00 USD Add to cart

In Stock Stock on request Limited stock

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