Amersham HCPQuant CHO ELISA Kit and Amersham Anti-CHO HCP Antibody | Cytiva, formerly GE Healthcare Life Sciences

In control: Do not let HCPs hold you back.

Amersham HCPQuant CHO ELISA and Amersham Anti-CHO HCP Antibody products reduce the risk of unexpected host cell proteins during the transition from generic to specific HCP ELISAs and help maximize the efficiency of your HCP management strategy.

Wide dynamic range and strong dilutional linearity to increase confidence across the entire purification process
Sensitive antibody with strong HCP coverage to minimize undetected HCPs
Consistently low inter- and intra-plate variation to ensure reproducible data

Confidence in results

Dilutional linearity throughout purification indicates good sample compatibility and data reliability.

Calculating the same stock concentration of HCP across several dilutions of the same sample is known as parallelism. Parallelism indicates a robust assay that optimizes processes and saves time and resources. Here, we demonstrate sample compatibility and parallelism for CHO-S.

Why challenge your ELISA?

Challenging your assay for each new molecule is essential to selecting the most suitable kit.

We used two different kits to test nine commercial biologics for residual HCP.

Cytiva: Amersham HCPQuant CHO (Supernatant) Kit reported higher HCP in four samples.
Cygnus Technologies: CHO HCP ELISA 3G (F550) Kit reported higher HCP in two samples.
Both kits reported similar HCP in three samples.

Avoid unexpected HCP levels in later development.

We calculated the lower limit of detection (LLD) as the concentration where the signal is greater than three standard deviations from the mean of the zero standard [0.106 + (3 × 0.003) = 0.115].

We calculated the lower limit of quantification (LLQ) as the lowest concentration for which coefficient of variation (CV) is < 20% and recovery is between 80% and 120%. We determined the LLD and LLQ from eight replicates across two plates.

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Reproducibility for robust assays and confidence in your results

We spiked the assay with three sample concentrations to perform precision analysis. We calculated intra-assay precision from 10 replicates for each concentration, and calculated inter-assay precision from the averaged means of three replicates (three plates).

Inter-plate CV < 6.72%

Intra-plate CV < 4.7%

Amersham Anti-CHO HCP Antibody

If you leverage different HCP management strategies in process development, standard commercial kits might not meet your needs.

Cytiva offers antibodies for supernatant in stand-alone kits to enable better efficiency and flexibility in your lab processes.

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Product	Product Name	Pack size All 1 mg 1 unit 100 µg	Format All Tube 96 well plate 2 kits	Price
29496737	Amersham HCPQuant CHO (Supernatant)	1 unit	96 well plate	795.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
29496739	Amersham Anti-CHO HCP Antibody (Supernatant)	100 µg	Tube	On Request	Get Quote
29496740	Amersham Anti-CHO HCP Antibody (Supernatant)	1 mg	Tube	On Request	Get Quote
29613962	Amersham DIBE HCP Detection Kit: ECL PLEX G-A-R IGG, CY5 (150 µg); CYDYE DIGE CY3 MINIMAL (5 NMol)	1 unit	2 kits	550.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock

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HCPQuant CHO Kit and Anti-CHO HCP Antibody

In control: Do not let HCPs hold you back.

Confidence in results

Why challenge your ELISA?

Avoid unexpected HCP levels in later development.

Reproducibility for robust assays and confidence in your results

Amersham Anti-CHO HCP Antibody

Refine

Leave a Review

HCPQuant CHO Kit and Anti-CHO HCP Antibody

In control: Do not let HCPs hold you back.

Confidence in results

Why challenge your ELISA?

Avoid unexpected HCP levels in later development.

Reproducibility for robust assays and confidence in your results

Amersham Anti-CHO HCP Antibody

Select your model

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Leave a Review