Comprehensive HCP analysis workflow support

The typical image pair used for coverage assessment consists of the primary image, representing the total protein content of the HCP-antigen, and the secondary image, which reveals the proteins detected with the anti-HCP antibody. These images are generated from a host of applications using 2D PAGE-based methods, notably:

• Conventional 2D gel electrophoresis followed by 2D Western blotting
• 2D Differential In Blot Electrophoresis (2D DIBE) CyDye based assay
• Antibody affinity chromatography followed by 2D DIGE

Simple workflow

Melanie Coverage guides you through the image analysis process with a user-friendly step-by-step workflow, delivering quick results.

- Quality Control
- Alignment
- Detection
- Spot edition
- Presence filter
- Coverage

Apply efficient alignment strategies

Melanie enables increased alignment efficiency and minimizes match editing work by defining an appropriate alignment strategy. This avoids aligning all images to a single reference that might be quite dissimilar to many gels in the data set and would generate an unnecessarily large number of difficult alignments.

Use free viewer functionality

Please download the Melanie Coverage 9.2 analysis software package (200 MB) for Melanie. The software can open tutorial files and can be operated without licenses. To enable full functionality, contact us for a 21-day trial demo license.

Learn more about Cytiva's end-to-end solution for HCP analysis.