AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star or ECF detection can be used.
- Probes are labelled during a 30 minute reaction, resulting in probes that can be used without purification.
- Hybridization stringency can be controlled by temperature as well as salt concentration. Detection is rapid, taking only 30 minutes from completion of hybridization to application of detection reagent.
Each system includes the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500 cm² or 5000 cm² membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star or ECF detection reagents.
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