Perform a wide range of protein analysis applications

Melanie supports common detection agents, including fluorescence, colorimetric, and functional group-specific stains. Well suited for multiplex protein analysis for protein expression profiling and other applications.

Apply advanced normalization options

Use normalization when protein expression levels might vary between samples. Apply these options to host cells expressing recombinant protein or to analyze different subcellular fractions. This capability opens up new applications, from protein expression profiling to monoclonal antibody process development.

Fully exploit your experimental design information

Melanie‘s experimental design wizard helps you describe common one- and two-factor designs and assign images to a factor level. Then, use the design structure throughout analysis to propose specific alignment strategies, image display layouts, and appropriate statistical tests and data analysis tools. Principal component analysis (PCA) is supported.

Apply efficient alignment strategies

Melanie enables increased alignment efficiency and minimizes match editing work by defining an appropriate alignment strategy. This avoids aligning all images to a single reference that might be quite dissimilar to many gels in the data set and would generate an unnecessarily large number of difficult alignments.

Which software do I need?

Choose Melanie 9 Classic to perform visualization, matching, detection, quantitation, and analysis of 2-D gel electrophoresis images.

Choose Melanie 9 DIGE for 2D-DIGE and conventional 2-D gel analysis, Western blots, and other multiplex protein applications.

To upgrade from Classic to DIGE, choose the Melanie 9 DIGE upgrade package.

Use free viewer functionality

Please download the 2-D gel analysis software package (200 MB) for Melanie. The software can open tutorial files and can be operated without licenses. To enable full functionality, contact us for a 21-day trial demo license.

Learn more about Cytiva's end-to-end solution for HCP analysis.