2-D Quant Kit is designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques such as 2-D electrophoresis, SDS-PAGE, or IEF.

  • Accurately determine protein concentration in the presence of 2% SDS, 1% DTT, 8 M urea, 2 M thiourea, 4% CHAPS, 2% Pharmalyte, and 2% IPG Buffer.
  • Quantitatively precipitates proteins while leaving interfering substances behind.
  • Linear response in the range of 0 to 50 μg protein, with recommended sample volumes of 1 to 50 μl.

Common spectrophotometric methods of quantitating protein rely either on Coomassie dye binding or protein-catalyzed reduction of cupric (Cu2+) ion to cuprous (Cu+) ion. Dye-binding assays cannot be used in the presence of any reagent that also binds the dye. This includes carrier ampholytes and detergents such as PlusOne CHAPS, SDS, and Triton X-100. Assays that depend on the reduction of cupric ion cannot be used in the presence of reductants such as DTT, or in the presence of reagents that form complexes with cupric ion, such as thiourea or EDTA.

Read more
Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
Product Product Name Price
80648356 2-D Quant Kit 500 assays 423.00 USD Add to cart


No reviews have been left for this product. Be the first to leave a review!

Leave a Review

You are not logged in.

To leave a review Register or Log In

Looking for support assistance?

Explore Product Support like Accessories, FAQs, Flow path schemes, Maintenance, Packing procedures, Spare parts, Troubleshooting and more.

If you can't find what you need, contact us to solve your problems.

Contact Us